Dataset Information

Interplay between cadherins and ?2?1 integrin differentially regulates melanoma cell invasion.

ABSTRACT: Malignant transformation of melanocytes frequently coincides with an alteration in the expression of cell-cell adhesion molecules (cadherins) and cell-extracellular matrix proteins (integrins). How these two adhesion systems interplay to impact on cell invasion remains to be described in melanoma.Cell adhesion networks were localised by immunofluorescence in human primary cutaneous melanoma, metastatic melanoma in the lymph nodes, and melanoma cell lines. The role of these cell adhesion networks was assessed both in vivo, by analysing their impact on tumour growth in mice, and in vitro, with the use of functional tests including cell aggregation and cell migration.We found that ?2?1 integrin associates with both E-cadherin and N-cadherin to form two adhesive networks, distinguishable by the interaction-or not-of ?2?1 integrin with type I collagen. N-cadherin/?2?1 integrin and E-cadherin/?2?1 integrin networks differently participated towards tumour growth in mice. The N-cadherin/?2?1 integrin network showed specific involvement in melanoma cell invasion and migration towards type I collagen. On the other hand, the E-cadherin/?2?1 network regulated cell-cell adhesion.This suggests that different signalling environments can be generated, depending on the type and/or local concentration of cadherin present in the adhesion complex, which potentially leads to differential cell responses. Further clarification of how these adhesive networks are regulated is fundamental to understanding important physiological and pathological processes such as morphogenesis, wound healing, tumour invasion and metastasis.

SUBMITTER: Siret C

PROVIDER: S-EPMC4815890 | biostudies-other | 2015 Nov

REPOSITORIES: biostudies-other

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Interplay between cadherins and α2β1 integrin differentially regulates melanoma cell invasion.

Siret Carole C Terciolo Chloé C Dobric Aurelie A Habib Marie-Christine MC Germain Sebastien S Bonnier Renaté R Lombardo Dominique D Rigot Véronique V André Frédéric F

British journal of cancer 20151029 10

<h4>Background</h4>Malignant transformation of melanocytes frequently coincides with an alteration in the expression of cell-cell adhesion molecules (cadherins) and cell-extracellular matrix proteins (integrins). How these two adhesion systems interplay to impact on cell invasion remains to be described in melanoma.<h4>Methods</h4>Cell adhesion networks were localised by immunofluorescence in human primary cutaneous melanoma, metastatic melanoma in the lymph nodes, and melanoma cell lines. The r ...[more]

PMID: 26512877

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Dataset Information

Interplay between cadherins and ?2?1 integrin differentially regulates melanoma cell invasion.

Publications

Interplay between cadherins and α2β1 integrin differentially regulates melanoma cell invasion.

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