Ontology highlight
ABSTRACT:
SUBMITTER: Azad AF
PROVIDER: S-EPMC48171 | biostudies-other | 1992 Jan
REPOSITORIES: biostudies-other
Azad A F AF Sacci J B JB Nelson W M WM Dasch G A GA Schmidtmann E T ET Carl M M
Proceedings of the National Academy of Sciences of the United States of America 19920101 1
The identification of apparently fastidious microorganisms is often problematic. DNA from a rickettsia-like agent (called the ELB agent) present in cat fleas could be amplified by PCR with conserved primers derived from rickettsial 17-kDa common protein antigen and citrate synthase genes but not spotted fever group 190-kDa antigen gene. Alu I sites in both the 17-kDa and citrate synthase PCR products obtained with the rickettsia-like agent and Rickettsia typhi were different even though both age ...[more]