Ontology highlight
ABSTRACT:
SUBMITTER: Lal A
PROVIDER: S-EPMC4820846 | biostudies-other | 2016
REPOSITORIES: biostudies-other
Lal Avantika A Dhar Amlanjyoti A Trostel Andrei A Kouzine Fedor F Seshasayee Aswin S N AS Adhya Sankar S
Nature communications 20160330
DNA in bacterial cells primarily exists in a negatively supercoiled state. The extent of supercoiling differs between regions of the chromosome, changes in response to external conditions and regulates gene expression. Here we report the use of trimethylpsoralen intercalation to map the extent of supercoiling across the Escherichia coli chromosome during exponential and stationary growth phases. We find that stationary phase E. coli cells display a gradient of negative supercoiling, with the ter ...[more]