Effects of post-mortem and physical degradation on RNA integrity and quality.
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ABSTRACT:
SUBMITTER: Sidova M
PROVIDER: S-EPMC4822212 | biostudies-other | 2015 Sep
REPOSITORIES: biostudies-other
Publications
Effects of post-mortem and physical degradation on RNA integrity and quality.
Sidova Monika M Tomankova Silvie S Abaffy Pavel P Kubista Mikael M Sindelka Radek R
Biomolecular detection and quantification 20150821
The precision and reliability of quantitative nucleic acid analysis depends on the quality of the sample analyzed and the integrity of the nucleic acids. The integrity of RNA is currently primarily assessed by the analysis of ribosomal RNA, which is the by far dominant species. The extrapolation of these results to mRNAs and microRNAs, which are structurally quite different, is questionable. Here we show that ribosomal and some nucleolar and mitochondrial RNAs, are highly resistant to naturally ...[more]
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