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Annotation of the human cerebrospinal fluid lipidome using high resolution mass spectrometry and a dedicated data processing workflow.

ABSTRACT: Due to its proximity with the brain, cerebrospinal fluid (CSF) could be a medium of choice for the discovery of biomarkers of neurological and psychiatric diseases using untargeted analytical approaches.This study explored the CSF lipidome in order to generate a robust mass spectral database using an untargeted lipidomic approach.Cerebrospinal fluid samples from 45 individuals were analyzed by liquid chromatography coupled to high-resolution mass spectrometry method (LC-HRMS). A dedicated data processing workflow was implemented using XCMS software and adapted filters to select reliable features. In addition, an automatic annotation using an in silico lipid database and several MS/MS experiments were performed to identify CSF lipid species.Using this complete workflow, 771 analytically relevant monoisotopic lipid species corresponding to 550 unique lipids which represent five major lipid families (i.e., free fatty acids, sphingolipids, glycerophospholipids, glycerolipids, and sterol lipids) were detected and annotated. In addition, MS/MS experiments enabled to improve the annotation of 304 lipid species. Thanks to LC-HRMS, it was possible to discriminate between isobaric and also isomeric lipid species; and interestingly, our study showed that isobaric ions represent about 50 % of the total annotated lipid species in the human CSF.This work provides an extensive LC/HRMS database of the human CSF lipidome which constitutes a relevant foundation for future studies aimed at finding biomarkers of neurological disorders.

SUBMITTER: Seyer A

PROVIDER: S-EPMC4824825 | biostudies-other | 2016

REPOSITORIES: biostudies-other

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Annotation of the human cerebrospinal fluid lipidome using high resolution mass spectrometry and a dedicated data processing workflow.

Seyer Alexandre A Boudah Samia S Broudin Simon S Junot Christophe C Colsch Benoit B

Metabolomics : Official journal of the Metabolomic Society 20160407

<h4>Introduction</h4>Due to its proximity with the brain, cerebrospinal fluid (CSF) could be a medium of choice for the discovery of biomarkers of neurological and psychiatric diseases using untargeted analytical approaches.<h4>Objectives</h4>This study explored the CSF lipidome in order to generate a robust mass spectral database using an untargeted lipidomic approach.<h4>Methods</h4>Cerebrospinal fluid samples from 45 individuals were analyzed by liquid chromatography coupled to high-resolutio ...[more]

PMID: 27110228

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Dataset Information

Annotation of the human cerebrospinal fluid lipidome using high resolution mass spectrometry and a dedicated data processing workflow.

Publications

Annotation of the human cerebrospinal fluid lipidome using high resolution mass spectrometry and a dedicated data processing workflow.

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