Classification of fungal chitin synthases.
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ABSTRACT:
SUBMITTER: Bowen AR
PROVIDER: S-EPMC48270 | biostudies-other | 1992 Jan
REPOSITORIES: biostudies-other
Publications
Classification of fungal chitin synthases.
Bowen A R AR Chen-Wu J L JL Momany M M Young R R Szaniszlo P J PJ Robbins P W PW
Proceedings of the National Academy of Sciences of the United States of America 19920101 2
Comparison of the chitin synthase genes of Saccharomyces cerevisiae CHS1 and CHS2 with the Candida albicans CHS1 gene (UDP-N-acetyl-D-glucosamine:chitin 4-beta-N-acetylglucosaminyltransferase, EC 2.4.1.16) revealed two small regions of complete amino acid sequence conservation that were used to design PCR primers. Fragments homologous to chitin synthase (approximately 600 base pairs) were amplified from the genomic DNA of 14 fungal species. These fragments were sequenced, and their deduced amino ...[more]
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