Ontology highlight
ABSTRACT:
SUBMITTER: Hellman LM
PROVIDER: S-EPMC4837003 | biostudies-other | 2016 May
REPOSITORIES: biostudies-other
Hellman Lance M LM Yin Liusong L Wang Yuan Y Blevins Sydney J SJ Riley Timothy P TP Belden Orrin S OS Spear Timothy T TT Nishimura Michael I MI Stern Lawrence J LJ Baker Brian M BM
Journal of immunological methods 20160218
Measurements of thermal stability by circular dichroism (CD) spectroscopy have been widely used to assess the binding of peptides to MHC proteins, particularly within the structural immunology community. Although thermal stability assays offer advantages over other approaches such as IC50 measurements, CD-based stability measurements are hindered by large sample requirements and low throughput. Here we demonstrate that an alternative approach based on differential scanning fluorimetry (DSF) yiel ...[more]