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Thioflavin T as an efficient fluorescence sensor for selective recognition of RNA G-quadruplexes.

ABSTRACT: RNA G-quadruplexes (G4s) play important roles in translational regulation, mRNA processing events and gene expression. Therefore, a fluorescent probe that is capable of efficiently recognizing RNA G-quadruplex structures among other RNA forms is highly desirable. In this study, a water-soluble fluorogenic dye (i.e., Thioflavin T (ThT)) was employed to recognize RNA G-quadruplex structures using UV-Vis absorption spectra, fluorescence spectra and emission lifetime experiments. By stacking on the G-tetrad, the ThT probe exhibited highly specific recognition of RNA G-quadruplex structures with striking fluorescence enhancement compared with other RNA forms. The specific binding demonstrates that ThT is an efficient fluorescence sensor that can distinguish G4 and non-G4 RNA structures.

SUBMITTER: Xu S

PROVIDER: S-EPMC4838840 | biostudies-other | 2016 Apr

REPOSITORIES: biostudies-other

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Thioflavin T as an efficient fluorescence sensor for selective recognition of RNA G-quadruplexes.

Xu Shujuan S Li Qian Q Xiang Junfeng J Yang Qianfan Q Sun Hongxia H Guan Aijiao A Wang Lixia L Liu Yan Y Yu Lijia L Shi Yunhua Y Chen Hongbo H Tang Yalin Y

Scientific reports 20160421

RNA G-quadruplexes (G4s) play important roles in translational regulation, mRNA processing events and gene expression. Therefore, a fluorescent probe that is capable of efficiently recognizing RNA G-quadruplex structures among other RNA forms is highly desirable. In this study, a water-soluble fluorogenic dye (i.e., Thioflavin T (ThT)) was employed to recognize RNA G-quadruplex structures using UV-Vis absorption spectra, fluorescence spectra and emission lifetime experiments. By stacking on the ...[more]

PMID: 27098781

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