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Identification and synthesis of a major conserved antigenic epitope of Trypanosoma cruzi.

ABSTRACT: A gene sequence encoding an immunodominant protein with a repetitive epitope from the protozoan Trypanosoma cruzi, the causative agent of Chagas disease, was cloned and expressed. The identified 10-amino acid repeat is present within a high-molecular-weight trypomastigote antigen that appears specific to and conserved among T. cruzi isolates. More importantly, greater than 95% of T. cruzi infection sera, including both chronic and acute Chagas disease, contained elevated levels of antibody to a 15-amino acid synthetic peptide bearing the repetitive B-cell epitope. Considering the wide diversity of T. cruzi parasites, as well as the broad spectrum of clinical manifestations of Chagas disease, such a prevalent immune response among patients is significant and applicable to the control of Chagas disease through the diagnosis of T. cruzi infection.

SUBMITTER: Burns JM

PROVIDER: S-EPMC48424 | biostudies-other | 1992 Feb

REPOSITORIES: biostudies-other

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Identification and synthesis of a major conserved antigenic epitope of Trypanosoma cruzi.

Burns J M JM Shreffler W G WG Rosman D E DE Sleath P R PR March C J CJ Reed S G SG

Proceedings of the National Academy of Sciences of the United States of America 19920201 4

A gene sequence encoding an immunodominant protein with a repetitive epitope from the protozoan Trypanosoma cruzi, the causative agent of Chagas disease, was cloned and expressed. The identified 10-amino acid repeat is present within a high-molecular-weight trypomastigote antigen that appears specific to and conserved among T. cruzi isolates. More importantly, greater than 95% of T. cruzi infection sera, including both chronic and acute Chagas disease, contained elevated levels of antibody to a ...[more]

PMID: 1371355

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