Expression of IPT in Asakura-sanshoo (Zanthoxylum piperitum (L.) DC. f. inerme Makino) Alters Tree Architecture, Delays Leaf Senescence, and Changes Leaf Essential Oil Composition.

ABSTRACT: The IPT gene encodes isopentenyl pyrophosphate transferase, a key enzyme in cytokinin biosynthesis. We introduced IPT under the control of the CaMV35S promoter into Asakura-sanshoo (Zanthoxylum piperitum (L.) DC. f. inerme Makino) via stable Agrobacterium tumefaciens-mediated transformation. Three of 3-year-old transgenic Asakura-sanshoo lines Y5, Y16, and Y17 were used to evaluate the effects of IPT expression on the morphological characteristics, leaf senescence, and essential oil composition. Introduced IPT into Asakura-sanshoo stimulated an increase in cytokinin content and a decrease in auxin level. The increase in the cytokinin/auxin ratio affected the tree architecture in 3-year-old transgenic lines. The phenotypes of transgenic lines included reduced stem elongation, decreased leaf surface area, increased branching, and delayed leaf senescence. The expression of IPT in Asakura-sanshoo also affected the leaf essential oil composition. The amount of oxygenated sesquiterpenoid compounds in Y5 and Y16 was 21.1 and 15.8 % higher, respectively, than that in wild type (WT). The amount of aromatic compounds in Y5 and Y16 was 2.9 and 24.6 % lower, respectively, than that in WT. These results show that ipt expression in Asakura-sanshoo conferred desirable traits, including a dwarf growth habit, delayed senescence, and increased concentrations of some sesquiterpenoid compounds.

SUBMITTER: Zeng XF 

PROVIDER: S-EPMC4848336 | biostudies-other | 2016

REPOSITORIES: biostudies-other