Ontology highlight
ABSTRACT:
SUBMITTER: Temu T
PROVIDER: S-EPMC4848398 | biostudies-other | 2016 May
REPOSITORIES: biostudies-other
Temu Tikira T Mann Matthias M Räschle Markus M Cox Jürgen J
Bioinformatics (Oxford, England) 20160106 9
<h4>Unlabelled</h4>To enable mass spectrometry (MS)-based proteomic studies with poorly characterized organisms, we developed a computational workflow for the homology-driven assembly of a non-redundant reference sequence dataset. In the automated pipeline, translated DNA sequences (e.g. ESTs, RNA deep-sequencing data) are aligned to those of a closely related and fully sequenced organism. Representative sequences are derived from each cluster and joined, resulting in a non-redundant reference s ...[more]