Dataset Information

CCR5+T-bet+FoxP3+ Effector CD4 T Cells Drive Atherosclerosis.

ABSTRACT: CD4 T cells are involved in the pathogenesis of atherosclerosis, but atherosclerosis-specific CD4 T cells have not been described. Moreover, the chemokine(s) that regulates T-cell trafficking to the atherosclerotic lesions is also unknown.In Apoe(-/-) mice with mature atherosclerotic lesions (5 months of high fat diet), we find that most aortic T cells express CCR5 and interferon-? with a unique combination of cell surface markers (CD4(+)CD25(-)CD44(hi)CD62L(lo)) and transcription factors (FoxP3(+)T-bet(+)). We call these cells CCR5Teff. We investigated the role of CCR5 in regulating T-cell homing to the atherosclerotic aorta and the functionality of the CCR5Teff cells.CCR5Teff cells are exclusively found in the aorta and para-aortic lymph nodes of Apoe(-/-) mice. They do not suppress T-cell proliferation in vitro and are less potent than regulatory T cells at inhibiting cytokine secretion. Blocking or knocking out CCR5 or its ligand CCL5 significantly blocks T-cell homing to atherosclerotic aortas. Transcriptomic analysis shows that CCR5Teff cells are more similar to effector T cells than to regulatory T cells. They secrete interferon-?, interleukin-2, interleukin-10, and tumor necrosis factor. Adoptive transfer of these CCR5Teff cells significantly increases atherosclerosis.CCR5 is specifically needed for CD4 T-cell homing to the atherosclerotic plaques. CCR5(+)CD4 T cells express an unusual combination of transcription factors, FoxP3 and T-bet. Although CCR5Teff express FoxP3, we showed that they are not regulatory and adoptive transfer of these cells exacerbates atherosclerosis.

SUBMITTER: Li J

PROVIDER: S-EPMC4867125 | biostudies-other | 2016 May

REPOSITORIES: biostudies-other

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CCR5+T-bet+FoxP3+ Effector CD4 T Cells Drive Atherosclerosis.

Li Jie J McArdle Sara S Gholami Amin A Kimura Takayuki T Wolf Dennis D Gerhardt Teresa T Miller Jacqueline J Weber Christian C Ley Klaus K

Circulation research 20160328 10

<h4>Rationale</h4>CD4 T cells are involved in the pathogenesis of atherosclerosis, but atherosclerosis-specific CD4 T cells have not been described. Moreover, the chemokine(s) that regulates T-cell trafficking to the atherosclerotic lesions is also unknown.<h4>Objective</h4>In Apoe(-/-) mice with mature atherosclerotic lesions (5 months of high fat diet), we find that most aortic T cells express CCR5 and interferon-γ with a unique combination of cell surface markers (CD4(+)CD25(-)CD44(hi)CD62L(l ...[more]

PMID: 27021296

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Dataset Information

CCR5+T-bet+FoxP3+ Effector CD4 T Cells Drive Atherosclerosis.

Publications

CCR5+T-bet+FoxP3+ Effector CD4 T Cells Drive Atherosclerosis.

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