Ontology highlight
ABSTRACT:
SUBMITTER: Kozak S
PROVIDER: S-EPMC4869703 | biostudies-other | 2016 Apr
REPOSITORIES: biostudies-other
Kozak Sandra S Lercher Lukas L Karanth Megha N MN Meijers Rob R Carlomagno Teresa T Boivin Stephane S
Journal of biomolecular NMR 20160317 4
Maintaining a stable fold for recombinant proteins is challenging, especially when working with highly purified and concentrated samples at temperatures >20 °C. Therefore, it is worthwhile to screen for different buffer components that can stabilize protein samples. Thermal shift assays or ThermoFluor(®) provide a high-throughput screening method to assess the thermal stability of a sample under several conditions simultaneously. Here, we describe a thermal shift assay that is designed to optimi ...[more]