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K-sam gene encodes secreted as well as transmembrane receptor tyrosine kinase.

ABSTRACT: K-sam was first identified as a gene amplified in the stomach cancer cell line KATO-III. The size of the major transcript of the K-sam gene was 3.5 kilobases in KATO-III cells, and we have previously shown that K-sam encodes a receptor tyrosine kinase that belongs to the heparin-binding growth factor receptor, or fibroblast growth factor receptor, gene family. The K-sam gene expresses multiple sizes of mRNAs in brain tissue, the immature teratoma cell line NCC-IT, and KATO-III. RNA blot analyses with a variety of K-sam probes indicate that there are at least four classes of K-sam mRNAs. Three types of K-sam cDNAs in addition to the previously reported type of K-sam cDNA were isolated, and their nucleotide sequences encode a full-length transmembrane receptor, a secreted receptor with a tyrosine kinase domain, and a secreted receptor without a tyrosine kinase domain.

SUBMITTER: Katoh M

PROVIDER: S-EPMC48783 | biostudies-other | 1992 Apr

REPOSITORIES: biostudies-other

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K-sam gene encodes secreted as well as transmembrane receptor tyrosine kinase.

Katoh M M Hattori Y Y Sasaki H H Tanaka M M Sugano K K Yazaki Y Y Sugimura T T Terada M M

Proceedings of the National Academy of Sciences of the United States of America 19920401 7

K-sam was first identified as a gene amplified in the stomach cancer cell line KATO-III. The size of the major transcript of the K-sam gene was 3.5 kilobases in KATO-III cells, and we have previously shown that K-sam encodes a receptor tyrosine kinase that belongs to the heparin-binding growth factor receptor, or fibroblast growth factor receptor, gene family. The K-sam gene expresses multiple sizes of mRNAs in brain tissue, the immature teratoma cell line NCC-IT, and KATO-III. RNA blot analyses ...[more]

PMID: 1313574

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