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Human combinatorial antibody libraries to hepatitis B surface antigen.

ABSTRACT: Human antibody Fab fragments that bind to hepatitis B surface antigen (HBsAg) were generated by using a recombinant phage surface-display expression system. Characterization of HBsAg-specific Fab fragments isolated from two vaccinated individuals reveals diversity in specificity of antigen binding and in the sequences of the complementarity-determining region. The sequence results show examples of human light-chain promiscuity that result in fine specificity changes and a strong relationship to a human germ-line gene. This application illustrates further that this technique is a powerful tool to isolate distinct human antibodies against immunogenic viral targets.

SUBMITTER: Zebedee SL

PROVIDER: S-EPMC48828 | biostudies-other | 1992 Apr

REPOSITORIES: biostudies-other

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Human combinatorial antibody libraries to hepatitis B surface antigen.

Zebedee S L SL Barbas C F CF Hom Y L YL Caothien R H RH Graff R R DeGraw J J Pyati J J LaPolla R R Burton D R DR Lerner R A RA

Proceedings of the National Academy of Sciences of the United States of America 19920401 8

Human antibody Fab fragments that bind to hepatitis B surface antigen (HBsAg) were generated by using a recombinant phage surface-display expression system. Characterization of HBsAg-specific Fab fragments isolated from two vaccinated individuals reveals diversity in specificity of antigen binding and in the sequences of the complementarity-determining region. The sequence results show examples of human light-chain promiscuity that result in fine specificity changes and a strong relationship to ...[more]

PMID: 1373487

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