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Phospholipase D ? knock-out mutants are tolerant to severe drought stress.

ABSTRACT: Phospholipase D (PLD) is involved in different plant processes, ranging from responses to abiotic and biotic stress to plant development. Phospholipase D? (PLD?) is activated in dehydration and salt stress, producing the lipid second messenger phosphatidic acid. In this work we show that pld? Arabidopsis mutants were more tolerant to severe drought than wild-type plants. PLD? has been shown to be required for ABA regulation of stomatal closure of isolated epidermal peels. However, there was no significant difference in stomatal conductance at the whole plant level between wild-type and pld? mutants. Since PLD hydrolyses structural phospholipids, then we looked at membrane integrity. Ion leakage measurements showed that during dehydration of leaf discs pld? mutant has less membrane degradation compared to the wild-type. We further analyzed the mutants and showed that pld? have higher mRNA levels of RAB18 and RD29A compared to wild-type plants under normal growth conditions. Transient expression of AtPLD? in Nicotiana benthamiana plants induced a wilting phenotype. These findings suggest that, in wt plants PLD? disrupt membranes in severe drought stress and, in the absence of the protein (PLD? knock-out) might drought-prime the plants, making them more tolerant to severe drought stress. The results are discussed in relation to PLD? role in guard cell signaling and drought tolerance.

SUBMITTER: Distefano AM

PROVIDER: S-EPMC4883880 | biostudies-other | 2015

REPOSITORIES: biostudies-other

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Phospholipase D δ knock-out mutants are tolerant to severe drought stress.

Distéfano Ayelen M AM Valiñas Matías A MA Scuffi Denise D Lamattina Lorenzo L Ten Have Arjen A García-Mata Carlos C Laxalt Ana M AM

Plant signaling & behavior 20150101 11

Phospholipase D (PLD) is involved in different plant processes, ranging from responses to abiotic and biotic stress to plant development. Phospholipase Dδ (PLDδ) is activated in dehydration and salt stress, producing the lipid second messenger phosphatidic acid. In this work we show that pldδ Arabidopsis mutants were more tolerant to severe drought than wild-type plants. PLDδ has been shown to be required for ABA regulation of stomatal closure of isolated epidermal peels. However, there was no s ...[more]

PMID: 26340512

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Project description:The growth and development of maize roots are closely related to drought tolerance. In order to clarify the molecular mechanisms of drought tolerance between different maize (Zea mays L.) varieties at the protein level, the isobaric tags for relative and absolute quantitation (iTRAQ) quantitative proteomics were used for the comparative analysis of protein expression in the seedling roots of the drought-tolerant Chang 7-2 and drought-sensitive TS141 maize varieties under 20% polyethylene glycol 6000 (PEG 6000)-simulated drought stress. We identified a total of 7723 differentially expressed proteins (DEPs), 1243 were significantly differentially expressed in Chang 7-2 following drought stress, 572 of which were up-regulated and 671 were down-regulated; 419 DEPs were identified in TS141, 172 of which were up-regulated and 247 were down-regulated. In Chang 7-2, the DEPs were associated with ribosome pathway, glycolysis/gluconeogenesis pathway, and amino sugar and nucleotide sugar metabolism. In TS141, the DEPs were associated with metabolic pathway, phenylpropanoid biosynthesis pathway, and starch and sucrose metabolism. Compared with TS141, the higher drought tolerance of Chang 7-2 root system was attributed to a stronger water retention capacity; the synergistic effect of antioxidant enzymes; the strengthen cell wall; the osmotic stabilization of plasma membrane proteins; the effectiveness of recycling amino acid; and an improvement in the degree of lignification. The common mechanisms of the drought stress response between the two varieties included: The promotion of enzymes in the glycolysis/gluconeogenesis pathway; cross-protection against the toxicity of aldehydes and ammonia; maintenance of the cell membrane stability. Based on the proteome sequencing information, the coding region sequences of eight DEP-related genes were analyzed at the mRNA level by quantitative real-time PCR (qRT-PCR). The findings of this study can inform the future breeding of drought-tolerant maize varieties.

Dataset Information

Phospholipase D ? knock-out mutants are tolerant to severe drought stress.

Publications

Phospholipase D δ knock-out mutants are tolerant to severe drought stress.

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