Ontology highlight
ABSTRACT:
SUBMITTER: Kohler C
PROVIDER: S-EPMC4889307 | biostudies-other | 2016
REPOSITORIES: biostudies-other
Köhler Carsten C Koalick Dennis D Fabricius Anja A Parplys Ann Christin AC Borgmann Kerstin K Pospiech Helmut H Grosse Frank F
Cell cycle (Georgetown, Tex.) 20160101 7
Cdc45 is an essential protein that together with Mcm2-7 and GINS forms the eukaryotic replicative helicase CMG. Cdc45 seems to be rate limiting for the initial unwinding or firing of replication origins. In line with this view, Cdc45-overexpressing cells fired at least twice as many origins as control cells. However, these cells displayed an about 2-fold diminished fork elongation rate, a pronounced asymmetry of replication fork extension, and an early S phase arrest. This was accompanied by H2A ...[more]