Ontology highlight
ABSTRACT:
SUBMITTER: Wu X
PROVIDER: S-EPMC4900541 | biostudies-other | 2016
REPOSITORIES: biostudies-other
Wu Xiaochun X Zhao Lingling L Chen Zhi Z Ji Xin X Qiao Xianfeng X Jin Yaping Y Liu Wei W
PloS one 20160609 6
The intracellular amino acid pool within lysosome is a signal that stimulates the nutrient-sensing mTORC1 signalling pathway. The signal transduction cascade has garnered much attention, but little is known about the sequestration of the signalling molecules within the lysosome. Using human HEK293 cells as a model, we found that suppression of the BHD syndrome gene FLCN reduced the leucine level in lysosome, which correlated with decreased mTORC1 activity. Both consequences could be reversed by ...[more]