Unknown

Dataset Information

0

Molecular cloning of the alpha-1 subunit of an omega-conotoxin-sensitive calcium channel.

ABSTRACT: Of the four major types of Ca channel described in vertebrate cells (designated T, L, N, and P), N-type Ca channels are unique in that they are found specifically in neurons, have been correlated with control of neurotransmitter release, and are blocked by omega-conotoxin, a neuropeptide toxin isolated from the marine snail Conus geographus. A set of overlapping cDNA clones were isolated and found to encode a Ca channel alpha-1 subunit, designated rbB-I. Polyclonal antiserum generated against a peptide from the rbB-I sequence selectively immunoprecipitates high-affinity 125I-labeled omega-conotoxin-binding sites from labeled rat forebrain membranes. PCR analysis shows that, like N-type Ca channels, expression of rbB-I is limited to the nervous system and neuronally derived cell lines. This brain Ca channel may mediate the omega-conotoxin-sensitive Ca influx required for neurotransmitter release at many synapses.

SUBMITTER: Dubel SJ 

PROVIDER: S-EPMC49228 | biostudies-other | 1992 Jun

REPOSITORIES: biostudies-other

Json Xml
altmetric image

Publications

Molecular cloning of the alpha-1 subunit of an omega-conotoxin-sensitive calcium channel.

Dubel S J SJ   Starr T V TV   Hell J J   Ahlijanian M K MK   Enyeart J J JJ   Catterall W A WA   Snutch T P TP  

Proceedings of the National Academy of Sciences of the United States of America 19920601 11

Of the four major types of Ca channel described in vertebrate cells (designated T, L, N, and P), N-type Ca channels are unique in that they are found specifically in neurons, have been correlated with control of neurotransmitter release, and are blocked by omega-conotoxin, a neuropeptide toxin isolated from the marine snail Conus geographus. A set of overlapping cDNA clones were isolated and found to encode a Ca channel alpha-1 subunit, designated rbB-I. Polyclonal antiserum generated against a  ...[more]

Similar Datasets

Unknown Expression cloning of TMEM16A as a calcium-activated chloride channel subunit.
| S-EPMC2651354 | biostudies-literature
Unknown Molecular diversity of the calcium channel alpha2delta subunit.
| S-EPMC6782206 | biostudies-literature
Unknown Human alpha-endosulfine, a possible regulator of sulfonylurea-sensitive KATP channel: molecular cloning, expression and biological properties.
| S-EPMC20985 | biostudies-literature
Unknown New Conotoxin SO-3 Targeting N-type Voltage-Sensitive Calcium Channels
| S-EPMC3663411 | biostudies-literature
Unknown Structure of a complex between a voltage-gated calcium channel beta-subunit and an alpha-subunit domain.
| S-EPMC3076333 | biostudies-literature
Unknown Rat brain expresses an alternatively spliced form of the dihydropyridine-sensitive L-type calcium channel alpha 2 subunit.
| S-EPMC48844 | biostudies-other
Unknown Cloning of the alpha 1 subunit of a voltage-dependent calcium channel expressed in pancreatic beta cells.
| S-EPMC48283 | biostudies-other
Unknown Atherosclerosis-related molecular alteration of the human CaV1.2 calcium channel alpha1C subunit.
| S-EPMC1636572 | biostudies-literature
Transcriptomics Calcium channel β3 subunit regulates ATP-dependent migration of dendritic cells
2023-09-27 | GSE224912 | GEO
Unknown Differential blockade of rat alpha3beta4 and alpha7 neuronal nicotinic receptors by omega-conotoxin MVIIC, omega-conotoxin GVIA and diltiazem.
| S-EPMC1760671 | biostudies-other