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Molecular cloning of the alpha-1 subunit of an omega-conotoxin-sensitive calcium channel.

ABSTRACT: Of the four major types of Ca channel described in vertebrate cells (designated T, L, N, and P), N-type Ca channels are unique in that they are found specifically in neurons, have been correlated with control of neurotransmitter release, and are blocked by omega-conotoxin, a neuropeptide toxin isolated from the marine snail Conus geographus. A set of overlapping cDNA clones were isolated and found to encode a Ca channel alpha-1 subunit, designated rbB-I. Polyclonal antiserum generated against a peptide from the rbB-I sequence selectively immunoprecipitates high-affinity 125I-labeled omega-conotoxin-binding sites from labeled rat forebrain membranes. PCR analysis shows that, like N-type Ca channels, expression of rbB-I is limited to the nervous system and neuronally derived cell lines. This brain Ca channel may mediate the omega-conotoxin-sensitive Ca influx required for neurotransmitter release at many synapses.

SUBMITTER: Dubel SJ 

PROVIDER: S-EPMC49228 | biostudies-other | 1992 Jun

REPOSITORIES: biostudies-other

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Molecular cloning of the alpha-1 subunit of an omega-conotoxin-sensitive calcium channel.

Dubel S J SJ   Starr T V TV   Hell J J   Ahlijanian M K MK   Enyeart J J JJ   Catterall W A WA   Snutch T P TP  

Proceedings of the National Academy of Sciences of the United States of America 19920601 11

Of the four major types of Ca channel described in vertebrate cells (designated T, L, N, and P), N-type Ca channels are unique in that they are found specifically in neurons, have been correlated with control of neurotransmitter release, and are blocked by omega-conotoxin, a neuropeptide toxin isolated from the marine snail Conus geographus. A set of overlapping cDNA clones were isolated and found to encode a Ca channel alpha-1 subunit, designated rbB-I. Polyclonal antiserum generated against a  ...[more]

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