Ontology highlight
ABSTRACT:
SUBMITTER: Wei H
PROVIDER: S-EPMC4935871 | biostudies-other | 2016
REPOSITORIES: biostudies-other
Wei Hua H Tang Suming S Hu Tianyu T Zhao Guojie G Guan Yifu Y
Scientific reports 20160707
Dumbbell probe (DP) attracts increasing interests in rolling circle amplification (RCA). A universal DP production method through cleavage-ligation of hairpin was proposed and optimized. The production is characterized by restriction endonuclease (RE)-induced cleavage ends ligation. It has the advantage of phosphorylation-free, splint-free and purification-free. To optimize designing, we found that the position of RE cleavage sequence in the stem and the primer position in the loop affected the ...[more]