Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Aspergillus, Scedosporium, and Fusarium spp. in the Australian Clinical Setting.
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ABSTRACT:
SUBMITTER: Sleiman S
PROVIDER: S-EPMC4963518 | biostudies-other | 2016 Aug
REPOSITORIES: biostudies-other
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Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Aspergillus, Scedosporium, and Fusarium spp. in the Australian Clinical Setting.
Sleiman Sue S Halliday Catriona L CL Chapman Belinda B Brown Mitchell M Nitschke Joanne J Lau Anna F AF Chen Sharon C-A SC
Journal of clinical microbiology 20160601 8
We developed an Australian database for the identification of Aspergillus, Scedosporium, and Fusarium species (n = 28) by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). In a challenge against 117 isolates, species identification significantly improved when the in-house-built database was combined with the Bruker Filamentous Fungi Library compared with that for the Bruker library alone (Aspergillus, 93% versus 69%; Fusarium, 84% versus 42%; and Scedos ...[more]
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