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A high-throughput in vitro ring assay for vasoactivity using magnetic 3D bioprinting.

ABSTRACT: Vasoactive liabilities are typically assayed using wire myography, which is limited by its high cost and low throughput. To meet the demand for higher throughput in vitro alternatives, this study introduces a magnetic 3D bioprinting-based vasoactivity assay. The principle behind this assay is the magnetic printing of vascular smooth muscle cells into 3D rings that functionally represent blood vessel segments, whose contraction can be altered by vasodilators and vasoconstrictors. A cost-effective imaging modality employing a mobile device is used to capture contraction with high throughput. The goal of this study was to validate ring contraction as a measure of vasoactivity, using a small panel of known vasoactive drugs. In vitro responses of the rings matched outcomes predicted by in vivo pharmacology, and were supported by immunohistochemistry. Altogether, this ring assay robustly models vasoactivity, which could meet the need for higher throughput in vitro alternatives.

SUBMITTER: Tseng H

PROVIDER: S-EPMC4967891 | biostudies-other | 2016

REPOSITORIES: biostudies-other

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A high-throughput in vitro ring assay for vasoactivity using magnetic 3D bioprinting.

Tseng Hubert H Gage Jacob A JA Haisler William L WL Neeley Shane K SK Shen Tsaiwei T Hebel Chris C Barthlow Herbert G HG Wagoner Matthew M Souza Glauco R GR

Scientific reports 20160801

Vasoactive liabilities are typically assayed using wire myography, which is limited by its high cost and low throughput. To meet the demand for higher throughput in vitro alternatives, this study introduces a magnetic 3D bioprinting-based vasoactivity assay. The principle behind this assay is the magnetic printing of vascular smooth muscle cells into 3D rings that functionally represent blood vessel segments, whose contraction can be altered by vasodilators and vasoconstrictors. A cost-effective ...[more]

PMID: 27477945

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Dataset Information

A high-throughput in vitro ring assay for vasoactivity using magnetic 3D bioprinting.

Publications

A high-throughput in vitro ring assay for vasoactivity using magnetic 3D bioprinting.

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