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In vitro selection and amplification protocols for isolation of aptameric sensors for small molecules.


ABSTRACT: We recently optimized a procedure that directly yields aptameric sensors for small molecules in so-called structure-switching format. The protocol has a high success rate, short time, and is sufficiently simple to be readily implemented in a non-specialist laboratory. We provide a stepwise guide to this selection protocol.

SUBMITTER: Yang KA 

PROVIDER: S-EPMC4981533 | biostudies-other | 2016 Aug

REPOSITORIES: biostudies-other

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