Ontology highlight
ABSTRACT:
SUBMITTER: Shropshire JD
PROVIDER: S-EPMC4991892 | biostudies-other | 2016
REPOSITORIES: biostudies-other
Shropshire J Dylan JD van Opstal Edward J EJ Bordenstein Seth R SR
PeerJ 20160809
Development of a Nasonia in vitrogerm-free rearing system in 2012 enabled investigation of Nasonia-microbiota interactions and real-time visualization of parasitoid metamorphosis. However, the use of antibiotics, bleach, and fetal bovine serum introduced artifacts relative to conventional rearing of Nasonia. Here, we optimize the germ-free rearing procedure by using filter sterilization in lieu of antibiotics and by removing residual bleach and fetal bovine serum. Comparison of these methods rev ...[more]