Single Synonymous Mutations in KRAS Cause Transformed Phenotypes in NIH3T3 Cells.
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ABSTRACT:
SUBMITTER: Waters AM
PROVIDER: S-EPMC5042562 | biostudies-other | 2016
REPOSITORIES: biostudies-other
Publications
Single Synonymous Mutations in KRAS Cause Transformed Phenotypes in NIH3T3 Cells.
Waters Andrew M AM Bagni Rachel R Portugal Franklin F Hartley James L JL
PloS one 20160929 9
Synonymous mutations in the KRAS gene are clustered at G12, G13, and G60 in human cancers. We constructed 9 stable NIH3T3 cell lines expressing KRAS, each with one of these synonymous mutations. Compared to the negative control cell line expressing the wild type human KRAS gene, all the synonymous mutant lines expressed more KRAS protein, grew more rapidly and to higher densities, and were more invasive in multiple assays. Three of the cell lines showed dramatic loss of contact inhibition, were ...[more]
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