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High sensitivity of gold nanoparticles co-doped with Gd2O3 mesoporous silica nanocomposite to nasopharyngeal carcinoma cells.

ABSTRACT: Nanoprobes for combined optical and magnetic resonance imaging have tremendous potential in early cancer diagnosis. Gold nanoparticles (AuNPs) co-doped with Gd2O3 mesoporous silica nanocomposite (Au/Gd@MCM-41) can produce pronounced contrast enhancement for T1 weighted image in magnetic resonance imaging (MRI). Here, we show the remarkably high sensitivity of Au/Gd@MCM-41 to the human poorly differentiated nasopharyngeal carcinoma (NPC) cell line (CNE-2) using fluorescence lifetime imaging (FLIM). The upconversion luminescences from CNE-2 and the normal nasopharyngeal (NP) cells (NP69) after uptake of Au/Gd@MCM-41 show the characteristic of two-photon-induced-radiative recombination of the AuNPs. The presence of the Gd(3+) ion induces a much shorter luminescence lifetime in CNE-2 cells. The interaction between AuNPs and Gd(3+) ion clearly enhances the optical sensitivity of Au/Gd@MCM-41 to CNE-2. Furthermore, the difference in the autofluorescence between CNE-2 and NP69 cells can be efficiently demonstrated by the emission lifetimes of Au/Gd@MCM-41 through the Forster energy transfers from the endogenous fluorophores to AuNPs. The results suggest that Au/Gd@MCM-41 may impart high optical resolution for the FLIM imaging that differentiates normal and high-grade precancers.

SUBMITTER: Wang H

PROVIDER: S-EPMC5046069 | biostudies-other | 2016 Oct

REPOSITORIES: biostudies-other

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High sensitivity of gold nanoparticles co-doped with Gd2O3 mesoporous silica nanocomposite to nasopharyngeal carcinoma cells.

Wang Hui H Zhang Songjin S Tian Xiumei X Liu Chufeng C Zhang Lei L Hu Wenyong W Shao Yuanzhi Y Li Li L

Scientific reports 20161003

Nanoprobes for combined optical and magnetic resonance imaging have tremendous potential in early cancer diagnosis. Gold nanoparticles (AuNPs) co-doped with Gd2O3 mesoporous silica nanocomposite (Au/Gd@MCM-41) can produce pronounced contrast enhancement for T1 weighted image in magnetic resonance imaging (MRI). Here, we show the remarkably high sensitivity of Au/Gd@MCM-41 to the human poorly differentiated nasopharyngeal carcinoma (NPC) cell line (CNE-2) using fluorescence ...[more]

PMID: 27694966

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Project description:Anaplastic thyroid carcinoma (ATC) is a highly aggressive tumor with a poor prognosis and a low median survival rate because of insufficient effective therapeutic modalities. Recently, mesoporous silica nanoparticles (MSNs) as a green non?toxic and safe nanomaterial have shown advantages to be a drug carrier and to modify the targeting group to the targeted therapy. To aim of the study was to explore the effects of MSNs co?loading with 17?allylamino?17?demethoxy?geldanamycin (17?AAG; HSP90 inhibitor) and 9?(6?aminopyridin?3?yl)?1?(3?(trifluoromethyl)phenyl)benzo[h][1,6]naphthyridin?2(1H)?one (Torin2; mTOR inhibitor) by targeting vascular endothelial growth factor receptor 2 (VEGFR2) on the viability of human anaplastic thyroid carcinoma FRO cells. The cytotoxicity of 17?AAG and Torin2 were analyzed by MTT assay. The possible synergistic antitumor effects between 17?AAG and Torin2 were evaluated by CompuSyn software. Flow cytometry was performed to assess the VEGFR2 targeting of (17?AAG+Torin2)@MSNs?anti?VEGFR2 ab and uptake by FRO cells. An ATC xenograft mouse model was established to assess the antitumor effect of (17?AAG+Torin2)@MSNs?anti?VEGFR2 ab in vivo. The results revealed that the combination of 17?AAG and Torin2 inhibited the growth of FRO cells more effectively compared with single use of these agents. Additionally, the synergistic antitumor effect appeared when concentration ratio of the two drugs was 1:1 along with total drug concentration greater than 0.52 µM. Furthermore, in an ATC animal model, it was revealed that the (17?AAG+Torin2)@MSNs?anti?VEGFR2 ab therapy modality could most effectively prolong the median survival time [39.5 days vs. 33.0 days (non?targeted) or 27.5 days (control)]. Compared to (17?AAG+Torin2)@MSNs, the (17?AAG+Torin2)@MSNs?anti?VEGFR2 ab could not only inhibit ATC cell growth but also prolong the median survival time of tumor?bearing mice in vivo and vitro more effectively, which may provide a new promising therapy for ATC.

Dataset Information

High sensitivity of gold nanoparticles co-doped with Gd2O3 mesoporous silica nanocomposite to nasopharyngeal carcinoma cells.

Publications

High sensitivity of gold nanoparticles co-doped with Gd<sub>2</sub>O<sub>3</sub> mesoporous silica nanocomposite to nasopharyngeal carcinoma cells.

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