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A putative ATP-dependent RNA helicase involved in Saccharomyces cerevisiae ribosome assembly.

ABSTRACT: We have isolated a cold-sensitive mutant of Saccharomyces cerevisiae in which there is a deficit of 60S ribosomal subunits. Cold sensitivity and the assembly defect are recessive and cosegregate, defining a single essential gene that we designated DRS1 (deficiency of ribosomal subunits). The wild-type DRS1 gene was cloned by complementation of the cold-sensitive phenotype of drs1. Sequence analysis reveals a high degree of similarity to a family of proteins that are thought to function as ATP-dependent RNA helicases. Pulse-chase analysis of ribosomal RNA synthesis and processing indicates that the drs1 mutant accumulates the 27S precursor of the mature 25S rRNA. These results suggest that, as in pre-mRNA splicing, RNA helicase activities are involved in ribosomal RNA processing.

SUBMITTER: Ripmaster TL

PROVIDER: S-EPMC50503 | biostudies-other | 1992 Dec

REPOSITORIES: biostudies-other

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A putative ATP-dependent RNA helicase involved in Saccharomyces cerevisiae ribosome assembly.

Ripmaster T L TL Vaughn G P GP Woolford J L JL

Proceedings of the National Academy of Sciences of the United States of America 19921201 23

We have isolated a cold-sensitive mutant of Saccharomyces cerevisiae in which there is a deficit of 60S ribosomal subunits. Cold sensitivity and the assembly defect are recessive and cosegregate, defining a single essential gene that we designated DRS1 (deficiency of ribosomal subunits). The wild-type DRS1 gene was cloned by complementation of the cold-sensitive phenotype of drs1. Sequence analysis reveals a high degree of similarity to a family of proteins that are thought to function as ATP-de ...[more]

PMID: 1454790

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