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Rectal 1% Tenofovir Gel Use Associates with Altered Epidermal Protein Expression.


ABSTRACT: Rectal use of a 1% tenofovir (TFV) gel is currently being evaluated for HIV prevention. While careful assessment of mucosal safety of candidate microbicides is a primary concern, tools to assess mucosal toxicity are limited. Mass spectrometry-based proteomics is a sensitive and high-throughput technique that can provide in-depth information on inflammation processes in biological systems. In this study, we utilized a proteomics approach to characterize mucosal responses in study participants involved in a phase 1 clinical trial of a rectal TFV-based gel. Project Gel was a phase 1 randomized (1:1), double-blind, multisite, placebo-controlled trial in which 24 participants received rectal TFV or a universal placebo [hydroxyethyl cellulose (HEC)] over a course of 8 daily doses. Rectal mucosal swabs were collected after 0, 1, and 8 doses and were analyzed by label-free tandem mass spectrometry. Differential protein expression was evaluated using a combination of paired (time-effects) and unpaired (across study arm) t-tests, and multivariate [least absolute shrinkage and selection operator (LASSO)] modeling. Within the TFV arm, 7% (17/249, p?

SUBMITTER: Romas L 

PROVIDER: S-EPMC5067863 | biostudies-other | 2016 Oct/Nov

REPOSITORIES: biostudies-other

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Rectal 1% Tenofovir Gel Use Associates with Altered Epidermal Protein Expression.

Romas Laura L   Birse Kenzie K   Mayer Kenneth H KH   Abou Max M   Westmacott Garrett G   Giguere Rebecca R   Febo Irma I   Cranston Ross D RD   Carballo-Diéguez Alex A   McGowan Ian I   Burgener Adam A  

AIDS research and human retroviruses 20160802 10-11


Rectal use of a 1% tenofovir (TFV) gel is currently being evaluated for HIV prevention. While careful assessment of mucosal safety of candidate microbicides is a primary concern, tools to assess mucosal toxicity are limited. Mass spectrometry-based proteomics is a sensitive and high-throughput technique that can provide in-depth information on inflammation processes in biological systems. In this study, we utilized a proteomics approach to characterize mucosal responses in study participants inv  ...[more]

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