Ontology highlight
ABSTRACT:
SUBMITTER: Ishibashi T
PROVIDER: S-EPMC50720 | biostudies-other | 1992 Dec
REPOSITORIES: biostudies-other
Ishibashi T T Bottaro D P DP Chan A A Miki T T Aaronson S A SA
Proceedings of the National Academy of Sciences of the United States of America 19921201 24
Using an expression cloning strategy, we isolated a cDNA encoding a human protein-tyrosine-phosphatase. Bacteria expressing the kinase domain of the keratinocyte growth factor receptor (bek/fibroblast growth factor receptor 2) were infected with a fibroblast cDNA library in a phagemid prokaryotic expression vector and screened with a monoclonal anti-phosphotyrosine antibody. Among several clones showing decreased anti-phosphotyrosine recognition, one displayed phosphatase activity toward the kin ...[more]