A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface.
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ABSTRACT:
SUBMITTER: Gupta GD
PROVIDER: S-EPMC5089374 | biostudies-other | 2015 Dec
REPOSITORIES: biostudies-other
Publications
A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface.
Gupta Gagan D GD Coyaud Étienne É Gonçalves João J Gonçalves João J Mojarad Bahareh A BA Liu Yi Y Wu Qianzhu Q Gheiratmand Ladan L Comartin David D Tkach Johnny M JM Cheung Sally W T SW Bashkurov Mikhail M Hasegan Monica M Knight James D JD Lin Zhen-Yuan ZY Schueler Markus M Hildebrandt Friedhelm F Moffat Jason J Gingras Anne-Claude AC Raught Brian B Pelletier Laurence L
Cell 20151201 6
The centrosome is the primary microtubule organizing center of the cells and templates the formation of cilia, thereby operating at a nexus of critical cellular functions. Here, we use proximity-dependent biotinylation (BioID) to map the centrosome-cilium interface; with 58 bait proteins we generate a protein topology network comprising >7,000 interactions. Analysis of interaction profiles coupled with high resolution phenotypic profiling implicates a number of protein modules in centriole dupli ...[more]
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