Dataset Information

Insights into the Regulation of Rhizosphere Bacterial Communities by Application of Bio-organic Fertilizer in Pseudostellaria heterophylla Monoculture Regime.

ABSTRACT: The biomass and quality of Pseudostellariae heterophylla suffers a significant decline under monoculture. Since rhizosphere miobiome plays crucial roles in soil health, deep pyrosequencing combined with qPCR was applied to characterize the composition and structure of soil bacterial community under monoculture and different amendments. The results showed compared with the 1st-year planted (FP), 2nd-year monoculture of P. heterophylla (SP) led to a significant decline in yield and resulted in a significant increase in Fusarium oxysporum but a decline in Burkholderia spp. Bio-organic fertilizer (MT) formulated by combining antagonistic bacteria with organic matter could significantly promote the yield by regulating rhizosphere bacterial community. However, organic fertilizer (MO) without antagonistic bacteria could not suppress Fusarium wilt. Multivariate statistics analysis showed a distinct separation between the healthy samples (FP and MT) and the unhealthy samples (SP and MO), suggesting a strong relationship between soil microbial community and plant performance. Furthermore, we found the application of bio-organic fertilizer MT could significantly increase the bacterial community diversity and restructure microbial community with relatively fewer pathogenic F. oxysporum and more beneficial Burkholderia spp. In conclusion, the application of novel bio-organic fertilizer could effectively suppress Fusarium wilt by enriching the antagonistic bacteria and enhancing the bacterial diversity.

SUBMITTER: Wu L

PROVIDER: S-EPMC5110535 | biostudies-other | 2016

REPOSITORIES: biostudies-other

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Insights into the Regulation of Rhizosphere Bacterial Communities by Application of Bio-organic Fertilizer in Pseudostellaria heterophylla Monoculture Regime.

Wu Linkun L Chen Jun J Wu Hongmiao H Qin Xianjin X Wang Juanying J Wu Yanhong Y Khan Muhammad U MU Lin Sheng S Xiao Zhigang Z Luo Xiaomian X Zhang Zhongyi Z Lin Wenxiong W

Frontiers in microbiology 20161116

The biomass and quality of Pseudostellariae heterophylla suffers a significant decline under monoculture. Since rhizosphere miobiome plays crucial roles in soil health, deep pyrosequencing combined with qPCR was applied to characterize the composition and structure of soil bacterial community under monoculture and different amendments. The results showed compared with the 1st-year planted (FP), 2nd-year monoculture of P. heterophylla (SP) led to a significant decline in yield and r ...[more]

PMID: 27899917

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Project description:Decomposed organic materials, in combination with plant growth-promoting bacteria (PGPB), are environmentally friendly and reduce synthetic fertilizer use in rice production. A bio-organic fertilizer (BoF) was prepared using kitchen waste (79%), chita-dhan (unfilled rice grain) biochar (15%), rock phosphate (5%), and a consortium of 10 PGPB (1%) to supplement 30% nitrogen and to replace triple superphosphate (TSP) fertilizer in rice production with an improvement of soil health. PGPB were local isolates and identified using 16S ribosomal RNA partial gene sequences as Bacillus mycoides, Proteus sp., Bacillus cereus, Bacillus subtilis, Bacillus pumilus, Paenibacillus polymyxa, and Paenibacillus spp. Isolates could fix N2 by 0.7-1.4 g kg-1, solubilize 0.1-1.2 g kg-1 phosphate, and produce 0.1-40 g kg-1 indoleacetic acid. The performance of BoF was evaluated by 16 field experiments and 18 farmers' field demonstration trials during the year 2017-2020 in different parts of Bangladesh. Performances of BoF were evaluated based on control (T1), full synthetic fertilizer dose of N, P, and K (T2), BoF (2 t ha-1) + 70% N as urea + 100% K as muriate of potash (T3), 70% N as urea + 100% P as TSP + 100% K as muriate of potash (T4), and 2 t ha-1 BoF (T5) treatments. At the research station, average grain yield improved by 10-13% in T3 compared with T2 treatment. Depending on seasons, higher agronomic N use efficiency (19-30%), physiological N use efficiency (8-18%), partial factor productivity (PFP)N (114-150%), recovery efficiency (RE)N (3-31%), N harvest index (HIN) (14-24%), agronomic P use efficiency (22-25%), partial factor productivity of P (9-12%), AREP (15-23%), and HIP (3-6%) were obtained in T3 compared with T2 treatment. Research results were reflected in farmers' field, and significant (P < 0.05) higher plant height, tiller, panicle, grain yield, partial factor productivity of N and P were obtained in the same treatment. Application of BoF improved soil organic carbon by 6-13%, along with an increased number of PGPB as compared with full synthetic fertilizer dose. In conclusion, tested BoF can be considered as a green technology to reduce 30% synthetic N and 100% TSP requirements in rice production with improved soil health.

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Dataset Information

Insights into the Regulation of Rhizosphere Bacterial Communities by Application of Bio-organic Fertilizer in Pseudostellaria heterophylla Monoculture Regime.

Publications

Insights into the Regulation of Rhizosphere Bacterial Communities by Application of Bio-organic Fertilizer in <i>Pseudostellaria heterophylla</i> Monoculture Regime.

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