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Genome editing in maize directed by CRISPR-Cas9 ribonucleoprotein complexes.

ABSTRACT: Targeted DNA double-strand breaks have been shown to significantly increase the frequency and precision of genome editing. In the past two decades, several double-strand break technologies have been developed. CRISPR-Cas9 has quickly become the technology of choice for genome editing due to its simplicity, efficiency and versatility. Currently, genome editing in plants primarily relies on delivering double-strand break reagents in the form of DNA vectors. Here we report biolistic delivery of pre-assembled Cas9-gRNA ribonucleoproteins into maize embryo cells and regeneration of plants with both mutated and edited alleles. Using this method of delivery, we also demonstrate DNA- and selectable marker-free gene mutagenesis in maize and recovery of plants with mutated alleles at high frequencies. These results open new opportunities to accelerate breeding practices in a wide variety of crop species.

SUBMITTER: Svitashev S

PROVIDER: S-EPMC5116081 | biostudies-other | 2016 Nov

REPOSITORIES: biostudies-other

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Genome editing in maize directed by CRISPR-Cas9 ribonucleoprotein complexes.

Svitashev Sergei S Schwartz Christine C Lenderts Brian B Young Joshua K JK Mark Cigan A A

Nature communications 20161116

Targeted DNA double-strand breaks have been shown to significantly increase the frequency and precision of genome editing. In the past two decades, several double-strand break technologies have been developed. CRISPR-Cas9 has quickly become the technology of choice for genome editing due to its simplicity, efficiency and versatility. Currently, genome editing in plants primarily relies on delivering double-strand break reagents in the form of DNA vectors. Here we report biolistic delivery of pre ...[more]

PMID: 27848933

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