Fosfomycin and rifampin disk diffusion tests for detection of Escherichia coli mutator strains.
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ABSTRACT: A simple method, using commercial disks to detect Escherichia coli mutator strains, is proposed. The breakpoint for detecting strains with a mutation frequency >/=5 x 10(-7) was established at >/=70 and >/=20 colonies in the inhibition zone of fosfomycin and rifampin disks, respectively, after seeding 100 microl of an overnight culture. Strains with <30 and <10 colonies in fosfomycin and rifampin inhibition zones are presumptively non-mutators.
SUBMITTER: Galan JC
PROVIDER: S-EPMC516308 | biostudies-other | 2004 Sep
REPOSITORIES: biostudies-other
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