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Follicular growth after xenotransplantation of cryopreserved/thawed human ovarian tissue in SCID mice: dynamics and molecular aspects.

ABSTRACT: To study the influence of xenotransplantation on follicular recruitment and growth in cryopreserved/thawed human ovarian tissue.Two 3-mm pieces of cryopreserved/thawed human ovarian tissue obtained from female cancer patients (n?=?11) were xenotransplanted into a subcutaneous neck pouch of 6-week-old ovarectomized SCID mice (n?=?33) for 4 (n?=?18) and 12 (n?=?15) weeks.Thirty-two out of 33 mice survived the entire observation periods. Graft recovery rate was 95.58 % (65 of 68 grafts). The percentages of primordial follicles after 4 weeks (P?

SUBMITTER: Ayuandari S

PROVIDER: S-EPMC5171895 | biostudies-other | 2016 Dec

REPOSITORIES: biostudies-other

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Follicular growth after xenotransplantation of cryopreserved/thawed human ovarian tissue in SCID mice: dynamics and molecular aspects.

Ayuandari Sarrah S Winkler-Crepaz Katharina K Paulitsch Monika M Wagner Cora C Zavadil Claudia C Manzl Claudia C Ziehr Stephanie C SC Wildt Ludwig L Hofer-Tollinger Susanne S

Journal of assisted reproduction and genetics 20160727 12

<h4>Purpose</h4>To study the influence of xenotransplantation on follicular recruitment and growth in cryopreserved/thawed human ovarian tissue.<h4>Method</h4>Two 3-mm pieces of cryopreserved/thawed human ovarian tissue obtained from female cancer patients (n = 11) were xenotransplanted into a subcutaneous neck pouch of 6-week-old ovarectomized SCID mice (n = 33) for 4 (n = 18) and 12 (n = 15) weeks.<h4>Result</h4>Thirty-two out of 33 mice survived the entire observation periods. Graft recovery ...[more]

PMID: 27465301

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Project description:ObjectiveAutograft transplantation of vitrified cortical ovarian tissue is an acceptable clinical technique for fertility preservation in women. Xenograft transplantation into animal models could be useful for evaluating the safety of human vitrified ovarian tissue. This study targeted to evaluate impact of vitrification on expression of the genes associated with folliculogenesis after xenograft transplantation of human vitrified ovarian tissue to γ-irradiated mice.Materials and methodsIn this experimental study, ovarian biopsies were gathered from six transsexual persons. The cortical section of ovarian biopsies was separated and chopped into small pieces. These pieces were randomly divided into vitrified and non-vitrified groups. In each group some pieces were considered as non-transplanted tissues and the others were transplanted to γ-irradiated female National Medical Research Institute (NMRI) mice. Before and after two weeks of xenograft transplantation, histological assessment and evaluation of the expression of folliculogenesisassociated genes (FIGLA, GDF-9, KL and FSHR) were performed in both vitrified and non-vitrified groups.ResultsPercentage of the normal follicles and expression of the all examined genes from transplanted and nontransplanted tissue were similar in both vitrified and non-vitrified groups (P>0.05). After transplantation, the normal follicle rate was significantly decreased and among the folliculogenesis-associated genes, expression of GDF-9 gene was significantly increased, rather than before transplantation in vitrified and non-vitrified tissues (P<0.05).ConclusionThe vitrification method using dimethyl solphoxide and ethylene glycol (EG) had no remarkable effect on the normal follicular rate and expression of folliculogenesis-associated genes after two weeks human ovarian tissue xenografting. In addition, transplantation process can cause a significant decrease in normal follicular rate and expression of GDF-9 gene.

Dataset Information

Follicular growth after xenotransplantation of cryopreserved/thawed human ovarian tissue in SCID mice: dynamics and molecular aspects.

Publications

Follicular growth after xenotransplantation of cryopreserved/thawed human ovarian tissue in SCID mice: dynamics and molecular aspects.

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