Ontology highlight
ABSTRACT:
SUBMITTER: Clair G
PROVIDER: S-EPMC5177886 | biostudies-other | 2016 Dec
REPOSITORIES: biostudies-other
Clair Geremy G Piehowski Paul D PD Nicola Teodora T Kitzmiller Joseph A JA Huang Eric L EL Zink Erika M EM Sontag Ryan L RL Orton Daniel J DJ Moore Ronald J RJ Carson James P JP Smith Richard D RD Whitsett Jeffrey A JA Corley Richard A RA Ambalavanan Namasivayam N Ansong Charles C
Scientific reports 20161222
Laser capture microdissection (LCM)-enabled region-specific tissue analyses are critical to better understand complex multicellular processes. However, current proteomics workflows entail several manual sample preparation steps and are challenged by the microscopic mass-limited samples generated by LCM, impacting measurement robustness, quantification and throughput. Here, we coupled LCM with a proteomics workflow that provides fully automated analysis of proteomes from microdissected tissues. B ...[more]