Isolation and functional expression of a mammalian prohormone processing enzyme, murine prohormone convertase 1.
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ABSTRACT:
SUBMITTER: Korner J
PROVIDER: S-EPMC52183 | biostudies-other | 1991 Aug
REPOSITORIES: biostudies-other
Publications
Isolation and functional expression of a mammalian prohormone processing enzyme, murine prohormone convertase 1.
Korner J J Chun J J Harter D D Axel R R
Proceedings of the National Academy of Sciences of the United States of America 19910801 15
We have combined gene cloning with an assay for prohormone biosynthesis and processing in Xenopus oocytes to identify the genes that encode mammalian prohormone processing enzymes. The coinjection of RNA encoding murine prohormone convertase 1 (mPC1), a mammalian endoprotease, along with proopiomelanocortin RNA into an oocyte results in the appropriate cleavage after paired basic residues in the proopiomelanocortin polyprotein necessary to generate corticotropin. The ability of mPC1 to generate ...[more]
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