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Physiological Remediation of Cobalt Ferrite Nanoparticles by Ferritin.

ABSTRACT: Metallic nanoparticles have been increasingly suggested as prospective therapeutic nanoplatforms, yet their long-term fate and cellular processing in the body is poorly understood. Here we examined the role of an endogenous iron storage protein - namely the ferritin - in the remediation of biodegradable cobalt ferrite magnetic nanoparticles. Structural and elemental analysis of ferritins close to exogenous nanoparticles within spleens and livers of mice injected in vivo with cobalt ferrite nanoparticles, suggests the intracellular transfer of degradation-derived cobalt and iron, entrapped within endogenous protein cages. In addition, the capacity of ferritin cages to accommodate and store the degradation products of cobalt ferrite nanoparticles was investigated in vitro in the acidic environment mimicking the physiological conditions that are present within the lysosomes. The magnetic, colloidal and structural follow-up of nanoparticles and proteins in the lysosome-like medium confirmed the efficient remediation of nanoparticle-released cobalt and iron ions by ferritins in solution. Metal transfer into ferritins could represent a quintessential process in which biomolecules and homeostasis regulate the local degradation of nanoparticles and recycle their by-products.

SUBMITTER: Volatron J

PROVIDER: S-EPMC5220348 | biostudies-other | 2017 Jan

REPOSITORIES: biostudies-other

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Physiological Remediation of Cobalt Ferrite Nanoparticles by Ferritin.

Volatron Jeanne J Kolosnjaj-Tabi Jelena J Javed Yasir Y Vuong Quoc Lam QL Gossuin Yves Y Neveu Sophie S Luciani Nathalie N Hémadi Miryana M Carn Florent F Alloyeau Damien D Gazeau Florence F

Scientific reports 20170109

Metallic nanoparticles have been increasingly suggested as prospective therapeutic nanoplatforms, yet their long-term fate and cellular processing in the body is poorly understood. Here we examined the role of an endogenous iron storage protein - namely the ferritin - in the remediation of biodegradable cobalt ferrite magnetic nanoparticles. Structural and elemental analysis of ferritins close to exogenous nanoparticles within spleens and livers of mice injected in vivo with cobalt ferrite nanop ...[more]

PMID: 28067263

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Project description:The "green" synthesis of magnetite and cobalt ferrite nanoparticles (Fe3O4-NPs and CoFe2O4-NPs) using extracts of Artemisia annua L "hairy" roots was proposed. In particular, the effect and role of important variables in the 'green' synthesis process, including the metal-salt ratio, various counter ions in the reaction mixture, concentration of total flavonoids and reducing power of the extract, were evaluated. The morphology and size distribution of the magnetic nanoparticles (MNPs) depended on the metal oxidation state and ratio of Fe(iii) : Fe(ii) in the initial reaction mixture. MNPs obtained from divalent metal salts in the reaction mixture were non-uniform in size with high aggregation level. Samples obtained by the FeCl3/FeSO4 mixture with a ratio of Fe(iii) : Fe(ii) = 1 : 2 showed an irregular shape of the nanoparticles and high aggregation level. MNPs obtained by the FeCl3/FeSO4/CoCl2 mixture showed a regular shape with slight aggregation, and were in the nanosize range (10-17 nm). Thus, this mixture as a metal-precursor was used for MNP biosynthesis in the subsequent experiments. The XRD data showed that the magnetic specimens contained mainly spinel type phase. The data of EDX and XPS analysis indicated that the product of the "green" synthesis was magnetite with some impurities, owing to the obtained ratio of Fe : O being similar to the theoretical atomic ratio of magnetite (3 : 4). The Fe3O4-NP samples were superparamagnetic with high magnetization (until 68 emu g-1). The Co-containing MNPs demonstrated low ferromagnetic properties. The MNPs with pure magnetite phase, very good magnetization and uniform size distribution (ca. 12-14 nm) were prepared by the "hairy" root extract characterized by the highest amount of total flavonoids. According to the FTIR data, the synthesized Fe3O4-NPs had a core-shell like structure, in which the core was composed of Fe3O4, and the shell was formed by bioactive molecules. The presence of several organic compounds (such as flavonoids or carboxylic acids) plays a key role in the suppression of Fe3O4-NP aggregation without addition of a stabilizing agents. Synthesized Fe3O4-NP samples effectively removed Cu(ii) and Cd(ii) with the maximum adsorption capacity, reaching 29.9 mg g-1 and 33.5 mg g-1, respectively. It is probable that the presence of organic components in extracts plays an important role in the adsorption properties of biosynthesised MNPs. The obtained MNPs were successfully applied to the removal of heavy metal ions in the environmental water samples. Fe3O4-NPs also negatively affected plant growth in the case of using "hairy" roots as a test model, and the greatest inhibitory activity (99.56 wt%) was possessed by MNPs with high magnetic properties.

Dataset Information

Physiological Remediation of Cobalt Ferrite Nanoparticles by Ferritin.

Publications

Physiological Remediation of Cobalt Ferrite Nanoparticles by Ferritin.

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