Ontology highlight
ABSTRACT:
SUBMITTER: Lurick A
PROVIDER: S-EPMC5231900 | biostudies-other | 2017 Jan
REPOSITORIES: biostudies-other
Lürick Anna A Gao Jieqiong J Kuhlee Anne A Yavavli Erdal E Langemeyer Lars L Perz Angela A Raunser Stefan S Ungermann Christian C
Molecular biology of the cell 20161116 2
Membrane fusion at endomembranes requires cross-talk between Rab GTPases and tethers to drive SNARE-mediated lipid bilayer mixing. Several tethers have multiple Rab-binding sites with largely untested function. Here we dissected the lysosomal HOPS complex as a tethering complex with just two binding sites for the Rab7-like Ypt7 protein to determine their relevance for fusion. Using tethering and fusion assays combined with HOPS mutants, we show that HOPS-dependent fusion requires both Rab-bindin ...[more]