Molecular cloning and expression of human myocardial cGMP-inhibited cAMP phosphodiesterase.
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ABSTRACT:
SUBMITTER: Meacci E
PROVIDER: S-EPMC525562 | biostudies-other | 1992 May
REPOSITORIES: biostudies-other
Publications
Molecular cloning and expression of human myocardial cGMP-inhibited cAMP phosphodiesterase.
Meacci E E Taira M M Moos M M Smith C J CJ Movsesian M A MA Degerman E E Belfrage P P Manganiello V V
Proceedings of the National Academy of Sciences of the United States of America 19920501 9
We have cloned a cDNA for a myocardial cGMP-inhibited cAMP phosphodiesterase (cGI PDE) from a human heart cDNA library in lambda Zap II. The open reading frame [3.5 kilobases (kb)] of cDNA clone n.13.2 (7.7 kb) encodes a protein of 125 kDa. In Northern blots of total human ventricle RNA, a single mRNA species (8.3 kb) hybridized with a 4-kb EcoRI restriction fragment of clone n.13.2 cDNA (containing the entire open reading frame). The carboxyl-terminal region of the deduced amino acid sequence o ...[more]
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