Ontology highlight
ABSTRACT:
SUBMITTER: Rowles J
PROVIDER: S-EPMC52755 | biostudies-other | 1991 Nov
REPOSITORIES: biostudies-other
Rowles J J Slaughter C C Moomaw C C Hsu J J Cobb M H MH
Proceedings of the National Academy of Sciences of the United States of America 19911101 21
We have purified casein kinase I (CKI) over 6000-fold from bovine thymus and have sequenced seven tryptic peptides that account for nearly 25% of the primary sequence of the enzyme. By using PCR, partial cDNAs encoding CKI and a related enzyme (CKI-delta) were isolated. A product that may correspond to an alternatively spliced form of CKI was also detected. The CKI PCR product was used to probe a bovine brain cDNA library from which cDNAs corresponding to CKI (CKI-alpha) and two homologous enzym ...[more]