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Graphene Foam as a three-dimensional Platform for Myotube Growth.

ABSTRACT: This study demonstrates the growth and differentiation of C2C12 myoblasts into functional myotubes on 3-dimensional graphene foam bioscaffolds. Specifically, we establish both bare and laminin coated graphene foam as a biocompatible platform for muscle cells and identify that electrical coupling stimulates cell activity. Cell differentiation and functionality is determined by the expression of myotube heavy chain protein and Ca2+ fluorescence, respectively. Further, our data show that the application of a pulsed electrical stimulus to the graphene foam initiates myotube contraction and subsequent localized substrate movement of over 100 micrometers. These findings will further the development of advanced 3-dimensional graphene platforms for therapeutic applications and tissue engineering.

SUBMITTER: Krueger E

PROVIDER: S-EPMC5287265 | biostudies-other | 2016 Aug

REPOSITORIES: biostudies-other

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Graphene Foam as a three-dimensional Platform for Myotube Growth.

Krueger Eric E Chang A Nicole AN Brown Dale D Eixenberger Josh J Brown Raquel R Rastegar Sepideh S Yocham Katie M KM Cantley Kurtis D KD Estrada David D

ACS biomaterials science & engineering 20160624 8

This study demonstrates the growth and differentiation of C2C12 myoblasts into functional myotubes on 3-dimensional graphene foam bioscaffolds. Specifically, we establish both bare and laminin coated graphene foam as a biocompatible platform for muscle cells and identify that electrical coupling stimulates cell activity. Cell differentiation and functionality is determined by the expression of myotube heavy chain protein and Ca<sup>2+</sup> fluorescence, respectively. Further, our data show that ...[more]

PMID: 28164151

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