Unknown

Dataset Information

0

Platelet activating factor-induced expression of p21 is correlated with histone acetylation.


ABSTRACT: Ultraviolet (UV)-irradiated keratinocytes secrete the lipid mediator of inflammation, platelet-activating factor (PAF). PAF plays an essential role in UV-induced immune suppression and skin cancer induction. Dermal mast cell migration from the skin to the draining lymph nodes plays a prominent role in activating systemic immune suppression. UV-induced PAF activates mast cell migration by up-regulating mast cell CXCR4 surface expression. Recent findings indicate that PAF up-regulates CXCR4 expression via histone acetylation. UV-induced PAF also activates cell cycle arrest and disrupts DNA repair, in part by increasing p21 expression. Do epigenetic alterations play a role in p21 up-regulation??Here we show that PAF increases Acetyl-CREB-binding protein (CBP/p300) histone acetyltransferase expression in a time and dose-dependent fashion. Partial deletion of the HAT domain in the CBP gene, blocked these effects. Chromatin immunoprecipitation assays indicated that PAF-treatment activated the acetylation of the p21 promoter. PAF-treatment had no effect on other acetylating enzymes (GCN5L2, PCAF) indicating it is not a global activator of histone acetylation. This study provides further evidence that PAF activates epigenetic mechanisms to affect important cellular processes, and we suggest this bioactive lipid can serve as a link between the environment and the epigenome.

SUBMITTER: Damiani E 

PROVIDER: S-EPMC5291204 | biostudies-other | 2017 Feb

REPOSITORIES: biostudies-other

altmetric image

Publications

Platelet activating factor-induced expression of p21 is correlated with histone acetylation.

Damiani Elisabetta E   Puebla-Osorio Nahum N   Lege Bree M BM   Liu Jingwei J   Neelapu Sattva S SS   Ullrich Stephen E SE  

Scientific reports 20170203


Ultraviolet (UV)-irradiated keratinocytes secrete the lipid mediator of inflammation, platelet-activating factor (PAF). PAF plays an essential role in UV-induced immune suppression and skin cancer induction. Dermal mast cell migration from the skin to the draining lymph nodes plays a prominent role in activating systemic immune suppression. UV-induced PAF activates mast cell migration by up-regulating mast cell CXCR4 surface expression. Recent findings indicate that PAF up-regulates CXCR4 expres  ...[more]

Similar Datasets

| S-EPMC3404877 | biostudies-other
| S-EPMC6432867 | biostudies-literature
| S-EPMC2646610 | biostudies-literature
| S-EPMC1218232 | biostudies-other
| S-EPMC4656409 | biostudies-literature
2010-07-01 | E-GEOD-5545 | biostudies-arrayexpress
| S-EPMC6399872 | biostudies-other
| S-EPMC1137072 | biostudies-other
| S-EPMC2770739 | biostudies-literature
| S-EPMC4146846 | biostudies-literature