Ontology highlight
ABSTRACT:
SUBMITTER: Milagre I
PROVIDER: S-EPMC5300890 | biostudies-other | 2017 Jan
REPOSITORIES: biostudies-other
Milagre Inês I Stubbs Thomas M TM King Michelle R MR Spindel Julia J Santos Fátima F Krueger Felix F Bachman Martin M Segonds-Pichon Anne A Balasubramanian Shankar S Andrews Simon R SR Dean Wendy W Reik Wolf W
Cell reports 20170101 5
Global DNA demethylation is an integral part of reprogramming processes in vivo and in vitro, but whether it occurs in the derivation of induced pluripotent stem cells (iPSCs) is not known. Here, we show that iPSC reprogramming involves both global and targeted demethylation, which are separable mechanistically and by their biological outcomes. Cells at intermediate-late stages of reprogramming undergo transient genome-wide demethylation, which is more pronounced in female cells. Global demethyl ...[more]