Expanding the genetic code of Mus musculus.
Ontology highlight
ABSTRACT:
SUBMITTER: Han S
PROVIDER: S-EPMC5321798 | biostudies-other | 2017 Feb
REPOSITORIES: biostudies-other
Publications
Expanding the genetic code of Mus musculus.
Han Songmi S Yang Aerin A Lee Soonjang S Lee Han-Woong HW Park Chan Bae CB Park Hee-Sung HS
Nature communications 20170221
Here we report the expansion of the genetic code of Mus musculus with various unnatural amino acids including N<sup>ɛ</sup>-acetyl-lysine. Stable integration of transgenes encoding an engineered N<sup>ɛ</sup>-acetyl-lysyl-tRNA synthetase (AcKRS)/tRNA<sup>Pyl</sup> pair into the mouse genome enables site-specific incorporation of unnatural amino acids into a target protein in response to the amber codon. We demonstrate temporal and spatial control of protein acetylation in various organs of the t ...[more]
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