DNA as an environmental sensor: detection and identification of pesticide contaminants in water with fluorescent nucleobases.

ABSTRACT: Environmental contaminants pose a substantial health risk in many areas of the world. One of these risks is contamination of water with toxic organic species, such as herbicides and insecticides. Here we describe the discovery and properties of a set of fluorescent chemosensors that respond to micromolar concentrations of a broad range of common organic pesticides. The chemosensors are short DNA-like oligomers with fluorophores replacing DNA bases that are assembled via a DNA synthesizer. We screened a library of 1296 tetrameric compounds on polystyrene microbeads, and identified a set of chemosensor sequences that respond strongly to a set of structurally varied pesticide analytes. We show that ten chemosensors on beads can be used to detect and identify 14 different common pesticides at 100 ?M, using the pattern of fluorescence intensity and wavelength changes. Limits of detection for two analytes were as low as 2 ?M. The chemosensors are shown to function successfully in a practical setting, correctly identifying unknown pesticide contaminants in water from Felt Lake, California. The results establish a simple, low cost strategy for sensing environmental spills of toxic organics.

SUBMITTER: Kwon H 

PROVIDER: S-EPMC5322131 | biostudies-other | 2017 Feb

REPOSITORIES: biostudies-other