Ontology highlight
ABSTRACT:
SUBMITTER: Hession C
PROVIDER: S-EPMC53544 | biostudies-other | 1990 Mar
REPOSITORIES: biostudies-other
Hession C C Osborn L L Goff D D Chi-Rosso G G Vassallo C C Pasek M M Pittack C C Tizard R R Goelz S S McCarthy K K
Proceedings of the National Academy of Sciences of the United States of America 19900301 5
A cDNA for endothelial leukocyte adhesion molecule 1 (ELAM-1) was isolated by transient expression in COS-7 cells of a subtracted cDNA library from cytokine-treated human umbilical vein endothelial cells (HUVECs), with selection of ELAM-1-expressing clones by adhesion of transfected cells to the human promyelocytic cell line HL-60. This cloning method requires neither antibody nor purified ligand. ELAM-1-expressing COS cells bind the promyelocytic cell line HL-60 by a Ca2(+)-dependent but temper ...[more]