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Evidence for preferential genotyping of a minority human immunodeficiency virus population due to primer-template mismatching during PCR-based amplification.


ABSTRACT: Human immunodeficiency virus type 1 (HIV-1) genotyping assays have come to be widely used for monitoring antiretroviral drug resistance. We report a case in which primer-template mismatches during nested PCR-based amplification biased the composition of the original viral population in the sample, magnifying a distinct minority HIV-1 population. This observation might help to explain some unexpected HIV-1 genotypes.

SUBMITTER: Martinez-Picado J 

PROVIDER: S-EPMC540107 | biostudies-other | 2005 Jan

REPOSITORIES: biostudies-other

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