Ontology highlight
ABSTRACT:
SUBMITTER: Lee EH
PROVIDER: S-EPMC54168 | biostudies-other | 1990 Jun
REPOSITORIES: biostudies-other
Proceedings of the National Academy of Sciences of the United States of America 19900601 12
The Escherichia coli gene encoding protein n' has been isolated and named priA for primosomal protein A. Protein n' is absolutely required for the conversion of single-stranded phi X174 DNA to the duplex replicative form in an in vitro-reconstituted system. The gene maps to 88.7 minutes on the chromosome adjacent to the cytR locus. Soluble protein extracts from cells harboring the priA gene on a multicopy plasmid contained 45-fold more n' replication activity than wild-type extracts. Enhanced ov ...[more]