Ontology highlight
ABSTRACT:
SUBMITTER: Zhang Y
PROVIDER: S-EPMC5440813 | biostudies-other | 2017 May
REPOSITORIES: biostudies-other
Zhang Youjun Y Beard Katherine F M KFM Swart Corné C Bergmann Susan S Krahnert Ina I Nikoloski Zoran Z Graf Alexander A Ratcliffe R George RG Sweetlove Lee J LJ Fernie Alisdair R AR Obata Toshihiro T
Nature communications 20170516
Protein complexes of sequential metabolic enzymes, often termed metabolons, may permit direct channelling of metabolites between the enzymes, providing increased control over metabolic pathway fluxes. Experimental evidence supporting their existence in vivo remains fragmentary. In the present study, we test binary interactions of the proteins constituting the plant tricarboxylic acid (TCA) cycle. We integrate (semi-)quantitative results from affinity purification-mass spectrometry, split-lucifer ...[more]