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Confined-space synthesis of nanostructured anatase, directed by genetically engineered living organisms for lithium-ion batteries.

ABSTRACT: Biomineral formation processes in nature are temporally and spatially regulated under the functions of biomolecules in a confined space. It is potentially very productive to rationally design a mineralized system by taking into account confined space as well as biomolecules. The laboratory technique of "bacterial cell surface display" is an ideal platform to host catalytically active proteins in a three-dimensionally confined space. In the present study, aiming to regulate the synthesis of nanostructured TiO2 anatase, repeating segments of silaffin were displayed on Escherichia coli surfaces through genetic manipulation. The displayed protein electrostatically interacted with a titanium source and catalyzed the hydrolysis of titanium dioxide precursors through hydrogen bonding interactions on the cell surface. In the subsequent calcination process, the genetically modified cells not only served as a framework for producing rod-shaped TiO2 assembled by nanoparticles, but also provided a carbon source in situ. The size of nanoparticles was controlled by changing the number of tandem repeats of the protein segment. The as prepared TiO2 anatase exhibited unique characteristics including nanosized anatase crystals, mesoporous structure and carbon coating. When tested as the anode electrode of a lithium-ion battery, it showed excellent lithium storage performance. The carbon coated anatase anode shows a higher specific capacity of 207 mA h g-1 after 200 cycles at a current rate of 1C and an ultra-long cycling lifetime of 5000 cycles with an outstanding retention capacity of 149 mA h g-1 at a higher rate of 10C. This bioprocess-inspired approach may help broaden the scope and impact of nanosized biominerals.

SUBMITTER: Ping H

PROVIDER: S-EPMC5450446 | biostudies-other | 2016 Oct

REPOSITORIES: biostudies-other

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Confined-space synthesis of nanostructured anatase, directed by genetically engineered living organisms for lithium-ion batteries.

Ping Hang H Xie Hao H Xiang Mingyu M Su Bao-Lian BL Wang Yucheng Y Zhang Jinyong J Zhang Fan F Fu Zhengyi Z

Chemical science 20160714 10

Biomineral formation processes in nature are temporally and spatially regulated under the functions of biomolecules in a confined space. It is potentially very productive to rationally design a mineralized system by taking into account confined space as well as biomolecules. The laboratory technique of "bacterial cell surface display" is an ideal platform to host catalytically active proteins in a three-dimensionally confined space. In the present study, aiming to regulate the synthesis of nanos ...[more]

PMID: 28567245

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Project description:Gene drive organisms are a recent development created by using methods of genetic engineering; they inherit genetic constructs that are passed on to future generations with a higher probability than with Mendelian inheritance. There are some specific challenges inherent to the environmental risk assessment (ERA) of genetically engineered (GE) gene drive organisms because subsequent generations of these GE organisms might show effects that were not observed or intended in the former generations. Unintended effects can emerge from interaction of the gene drive construct with the heterogeneous genetic background of natural populations and/or be triggered by changing environmental conditions. This is especially relevant in the case of gene drives with invasive characteristics and typically takes dozens of generations to render the desired effect. Under these circumstances, "next generation effects" can substantially increase the spatial and temporal complexity associated with a high level of uncertainty in ERA. To deal with these problems, we suggest the introduction of a new additional step in the ERA of GE gene drive organisms that takes 3 criteria into account: the biology of the target organisms, their naturally occurring interactions with the environment (biotic and abiotic), and their intended biological characteristics introduced by genetic engineering. These 3 criteria are merged to form an additional step in ERA, combining specific "knowns" and integrating areas of "known unknowns" and uncertainties, with the aim of assessing the spatiotemporal controllability of GE gene drive organisms. The establishment of assessing spatiotemporal controllability can be used to define so-called "cut-off criteria" in the risk analysis of GE gene drive organisms: If it is likely that GE gene drive organisms escape spatiotemporal controllability, the risk assessment cannot be sufficiently reliable because it is not conclusive. Under such circumstances, the environmental release of the GE gene drive organisms would not be compatible with the precautionary principle (PP). Integr Environ Assess Manag 2020;00:1-14. © 2020 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals, Inc. on behalf of Society of Environmental Toxicology & Chemistry (SETAC).

Dataset Information

Confined-space synthesis of nanostructured anatase, directed by genetically engineered living organisms for lithium-ion batteries.

Publications

Confined-space synthesis of nanostructured anatase, directed by genetically engineered living organisms for lithium-ion batteries.

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